A review on columns used in chromatography

  • Badri.Sireesha Assistant Professor, Sankar Reddy Institute of Pharmaceutical Sciences, Salakalaveedu(V), Bestavaripeta (M), Prakasam (D), Pincode-523370
  • K.Shalini B.Pharmacy, Sankar Reddy Institute of Pharmaceutical Sciences, Salakalaveedu(V), Bestavaripeta (M), Prakasam (D), Pincode-523370
  • A.Janaki B.Pharmacy, Sankar Reddy Institute of Pharmaceutical Sciences, Salakalaveedu(V), Bestavaripeta (M), Prakasam (D), Pincode-523370
  • N.Sailaja B.Pharmacy, Sankar Reddy Institute of Pharmaceutical Sciences, Salakalaveedu(V), Bestavaripeta (M), Prakasam (D), Pincode-523370
  • G.Ishwarya B.Pharmacy, Sankar Reddy Institute of Pharmaceutical Sciences, Salakalaveedu(V), Bestavaripeta (M), Prakasam (D), Pincode-523370
  • M.Sai charan B.Pharmacy, Sankar Reddy Institute of Pharmaceutical Sciences, Salakalaveedu(V), Bestavaripeta (M), Prakasam (D), Pincode-523370
  • D.Lokesh kumar B.Pharmacy, Sankar Reddy Institute of Pharmaceutical Sciences, Salakalaveedu(V), Bestavaripeta (M), Prakasam (D), Pincode-523370
DOI https://doi.org/10.37022/tjmdr.v3i3.502

Abstract

Column chromatography is used extensively in nucleic acid chemistry to purify or characterize products from chemical reactions. This appendix focuses on separations using silica gel or alumina. The procedures described include loading the column, assembling the apparatus, separating compounds and collecting fractions, and optimizing chromatography conditions.Column chromatography issimple and the most popularseparation and purification technique. Column chromatography consists of a stationary solid phase that adsorbs and separates the compounds passing through it with the help of a liquid mobile phase. Various stationary phases, such as silica, alumina, calcium phosphate, calcium carbonate, starch, and magnesia, and different solvent compositions based on the nature of compounds to be separated and isolated, are used in column chromatography.

Keywords: Column, HPLC techniques, Separation, Absorption, Size exclusion

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Published
26/10/2023
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Badri, S., K, S., A, J., N, S., G, I., M, S. charan, & D, L. kumar. (2023). A review on columns used in chromatography. The Journal of Multidisciplinary Research, 3(3), 17-23. https://doi.org/10.37022/tjmdr.v3i3.502
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Volume-3, Issue-3, 2023
Section
Review Articles
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