A comprehensive review on hyphenated techniques

Abstract

The hyphenated technique refers to the combination (or) fusion of distinct analytical methodologies. Spectroscopic techniques are mostly paired with chromatographic techniques. After that, an interphase will allow the mixture's separated components from the chromatographic approach to enter the spectroscopic technique. In GC-MS The separated components from the gas chromatography process are introduced into the mass spectrometer, where they undergo ionization, mass analysis, and measurement of the mass-to-charge ratios of the ions produced by each analysis. GC and MS can be connected by a membrane separator, jet/orifice separator, or effusion separator. The analytical flow cell in LC-NMR coupling was originally designed for continuous flow to NMR. However, the application in the stopped-flow mode in LC-MS has resulted from the requirement for a thorough structural study of novel natural compounds. The use of LC-MS-MS is growing daily at a rapid rate. When used with biological screening, hyphenated techniques like mass spectrometry (LC-UV-MS) and HPLC coupled to UV have proven to be very helpful for a quick analysis of natural products. Currently, a wide range of LC-MS systems with diverse interface options are offered for sale. Hyphenated procedures are methods of separation and identification that demonstrate improved sample analysis through the use of specificity, accuracy, and precision as constituents.