Evaluation of Analgesic Activity and Phytochemical Screening of Methanolic Extract Stem Bark Extract of Dialium Guineense (Wild)
Abstract
Dialium guineense stem bark is widely distributed in many tropical regions and has long been utilized in traditional medicine for the management of various infectious and degenerative disorders. This study was conducted to investigate the phytochemical constituents of the ethanolic stem bark extract of Dialium guineense and to evaluate its analgesic potential in experimental rats. Fresh stem bark was collected, dried, pulverized, and extracted using ethanol. The resulting ethanolic extract was administered orally at doses of 100 mg/kg and 200 mg/kg body weight to assess its analgesic activity. Analgesic effects were evaluated using the hot-plate and tail-flick methods in albino Wistar rats. Diclofenac sodium served as the standard reference drug. The findings demonstrated that the ethanolic stem bark extract of Dialium guineense produced significant analgesic effects in both experimental models, indicating its potential as a natural analgesic agent.
Keywords:
Dialium guineense stem bark, ethanolic extract, hot-plate test, tail-flick test, analgesic activity
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3. Gnansounou SM, Robin M, Brunel JM, et al. Dialium guineense, Parkia biglobosa and Tamarindus indica: Review of known and synergistic bioactive compounds. Journal of Medicinal Plants Studies. 2018;6(3):103–111.
4. Alqahtani YS, et al. A comprehensive review of the chemical composition and pharmacological activities of the genus Dialium. Jordan Journal of Chemistry. 2024.
5. Bancos S, Bernard MP, Topham DJ, Phipps RP. Ibuprofen and other widely used NSAIDs inhibit antibody production in human cells. Cellular Immunology. 2019.
6. Furman D, Campisi J, Verdin E, et al. Chronic inflammation in the etiology of disease across the life span. Nature Medicine. 2019;25:1822–1832.
7. Medzhitov R. The spectrum of inflammatory responses. Science. 2021;374:1070–1075.
8. Chen L, Deng H, Cui H, et al. Inflammatory responses and inflammation-associated diseases in organs. Oncotarget. 2018;9:7204–7218.
9. Calder PC. Nutrition, immunity and inflammation. BMJ Nutrition, Prevention & Health. 2020;3:74–92.
10. Dinarello CA. Overview of the IL-1 family in innate inflammation and acquired immunity. Immunological Reviews. 2018.
11. Fullerton JN, Gilroy DW. Resolution of inflammation: a new therapeutic frontier. Nature Reviews Drug Discovery. 2019.
12. Vane JR, Botting RM. The mechanism of action of anti-inflammatory drugs revisited. Journal of Physiology and Pharmacology. 2019.
13. Ricciotti E, FitzGerald GA. Prostaglandins and inflammation. Arteriosclerosis, Thrombosis, and Vascular Biology. 2021.
14. Crofford LJ. Use of NSAIDs in treating patients with arthritis. Arthritis Research & Therapy. 2020.
15. Singh A, Zhao K. Treatment of pain with herbal medicines: a systematic review. Journal of Ethnopharmacology. 2021.
16. Salehi B, Sharifi-Rad J, et al. Medicinal plants with anti-inflammatory activities: A review. Frontiers in Pharmacology. 2019.
17. Sharifi-Rad M, et al. Natural products against inflammation: Current status and future perspectives. Phytotherapy Research. 2020.
18. Rodrigues T, Reker D, Schneider P, Schneider G. Natural products in drug discovery. Nature Chemistry. 2018.
19. Atanasov AG, Zotchev SB, et al. Natural products in drug discovery: Advances and opportunities. Nature Reviews Drug Discovery. 2021.
20. Newman DJ, Cragg GM. Natural products as sources of new drugs over nearly four decades. Journal of Natural Products. 2020.
21. Yoon WJ, et al. Plant-derived flavonoids as anti-inflammatory agents. Molecules. 2021.
22. Hussain T, et al. Oxidative stress and inflammation: What polyphenols can do for us? Oxidative Medicine and Cellular Longevity. 2020.
23. Choi JY, et al. Role of cyclooxygenase-2 in inflammatory diseases. International Journal of Molecular Sciences. 2021.
24. Yang Y, Kim SC, Yu T, et al. Functional roles of nitric oxide in inflammatory responses. International Journal of Molecular Sciences. 2022.
25. Li B, et al. Macrophage-mediated inflammation and therapeutic targets. Frontiers in Immunology. 2023.
26. Zhang JM, An J. Cytokines, inflammation and pain. International Anesthesiology Clinics. 2022.
27. Rajapaksha HKK, Fernando MN, Nelumdeniya NRM, et al. Evaluation of in vitro anti-inflammatory activity and molecular docking study of medicinal plant extracts. 2024.
Published
15/09/2026
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Thalla, S., & M, M. K. (2026). Evaluation of Analgesic Activity and Phytochemical Screening of Methanolic Extract Stem Bark Extract of Dialium Guineense (Wild). Journal of Innovations in Applied Pharmaceutical Science (JIAPS), 11(3), 11-13. https://doi.org/10.37022/jiaps.v11i3.975
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