In vitro screening of icacinaceous plants indigenous to kerala

  • Dr.Elizabeth Abraham P Professor, Department of Pharmacology, Nirmala College of Pharmacy Muvattupuzha, Kerala, India
  • Frinto Francis Department of Pharmaceutical Sciences (CPAS) Puthuppaly, Kottayam, India
  • Pradeep R Nair Department of Pharmaceutical Sciences (CPAS) Puthuppaly, Kottayam , India
  • Athul Raj Department of Pharmaceutical Sciences (CPAS) Puthuppaly, Kottayam , India
  • Raji Rajan Department of Pharmaceutical Sciences (CPAS) Puthuppaly, Kottayam , India
  • Anamika K. Nair Department of Pharmaceutical Sciences (CPAS) Puthuppaly, Kottayam , India
  • Prof.Dr.Badmanaban.R Principal ,Nirmala college of Pharmacy, Muvattupuzha, Kerala, India

Abstract

This project work was focused on “in vitro screening of Icacinaceous plants indigenous to Kerala”. From phytochemical analysis of ethyl acetate extract of leaves of Sarcostigma kleinii it was found that carbohydrates, alkaloids, flavonoids,tannins, phenols, glycosides are present and saponins,proteins & amino acids are absent. From phytochemical analysis of ethyl acetate extract of leaves of Pyrenacantha volubilis it was found that carbohydrates, proteins and amino acids, flavonoids,alkaloids, glycosides, tannins and phenolic compounds are present. Sarcostigma kleinii leaves are used to treat helminthiasis, ulcers, leprosy and skin conditions traditionally. Pyrenacantha volubilisleaves are mainly used to treat cancer. The present study deals with the anti-inflammatory activity and antibacterial activity of ethyl acetate extracts of leaves of Sarcostigma kleinii and Pyrenacantha volubilis of Icacinaceae family. The results suggest that ethyl acetate extract of leaves of these two Icacinaceous plants may serve as a anti - inflammatory and antibacterial agents which may be due to the chemical constituents like alkaloids, phenols, tannins or flavonoids present in these plants.

Keywords: Screening, in-vitro screening, antibacterial activity, anti-inflammatory, biochemical activity, laboratory models

Downloads

Download data is not yet available.

Author Biographies

Dr.Elizabeth Abraham P, Professor, Department of Pharmacology, Nirmala College of Pharmacy Muvattupuzha, Kerala, India

Professor, Department of Pharmacology, Nirmala College of Pharmacy Muvattupuzha, Kerala, India

Frinto Francis, Department of Pharmaceutical Sciences (CPAS) Puthuppaly, Kottayam, India

Department of Pharmaceutical Sciences (CPAS) Puthuppaly, Kottayam, India

Pradeep R Nair, Department of Pharmaceutical Sciences (CPAS) Puthuppaly, Kottayam , India

Department of Pharmaceutical Sciences (CPAS) Puthuppaly, Kottayam , India

Athul Raj, Department of Pharmaceutical Sciences (CPAS) Puthuppaly, Kottayam , India

Department of Pharmaceutical Sciences (CPAS) Puthuppaly, Kottayam , India

Raji Rajan, Department of Pharmaceutical Sciences (CPAS) Puthuppaly, Kottayam , India

Department of Pharmaceutical Sciences (CPAS) Puthuppaly, Kottayam , India.

Anamika K. Nair, Department of Pharmaceutical Sciences (CPAS) Puthuppaly, Kottayam , India

Department of Pharmaceutical Sciences (CPAS) Puthuppaly, Kottayam , India

Prof.Dr.Badmanaban.R, Principal ,Nirmala college of Pharmacy, Muvattupuzha, Kerala, India

Principal ,Nirmala college of Pharmacy, Muvattupuzha, Kerala, India

References

1. Petrovska B. Historical review of medicinal plants usage. PharmacognosyReviews. 2012;6(11):1.
2. Koparde A, Doijad C, Magdum S. Natural Products in Drug Discovery. In: Pharmacognosy - Medicinal Plants. IntechOpen; 2019;8(13):1-17.
3. Simonsen HT, Nordskjold JB, Smitt UW, Nyman U, Palpu P, Joshi P, et al. Invitro screening of Indian medicinal plants for antiplasmodial activity. Journal of Ethnopharmacology. 2001;74(2):195–04.
4. Phanse MA. In-vivo and in-vitro screening of medicinal plants for their anti-inflammatory activity: an overview. Journal of Applied Pharmaceutical Science. 2012;02(07):19-33.
5. Pahwa R, Goyal A, Jialal I. ChronicInflammation. In: Stat Pearls [Internet]. Treasure Island (FL): Stat Pearls Publishing; 2022.
6. Jogpal V, Sanduja M, Dutt R, Garg V, Tinku. Advancement of nanomedicines in chronic inflammatory disorders. Inflammopharmacology. 2022 Apr;30(2):355-68.
7. Ríos JL, Recio MC. Medicinal plants and antimicrobial activity. Journal of Ethnopharmacology. 2005;100(1–2):80–84.
8. Abraham E,Harindran J. LCMS analysis and in-vitro antidepressant study ofethyl alcoholic extract of shade dried leaves of Sarcostigma kleinii Wight & Arn (icacinaceae) . world journal of pharmacy and pharmaceutical sciences. 2019;8(12):600-08.
9. P K Warrier. Indian Medicinal Plants a compendium of 500 plants. Vol.5,Chennai: Orient Longman; 1996. 76–79 p.
10. Redfern J, Kinninmonth M, Burdass D, Verran J. Using Soxhlet Ethanol Extraction to Produce and Test Plant Material (Essential Oils) for Their Antimicrobial Properties. Journal of Microbiology & Biology Education.2014;15(1):45–46.
11. Shaikh JR, Patil M. Qualitative tests for preliminary phytochemical screening: An overview. International Journal of Chemical Studies. 2020;8(2):603–08.
12. Gul R, Jan SU, Faridullah S, Sherani S, Jahan N. Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan. TheScientific World Journal. 2017;2017:1–7.
13. Valgas C, Souza SM de, Smânia EFA, Smânia Jr. A. Screening methods to determine antibacterial activity of natural products. Brazilian Journal of Microbiology. 2007;38(2):369–80.
14. Huys G, D’Haene K, Swings J. Influence of the culture medium on antibiotic susceptibility testing of food-associated lactic acid bacteria with the agar overlay disc diffusion method. Letters in Applied Microbiology. 2002;34(6):402–06.
15. Chandra S, Chatterjee P, Dey P, Bhattacharya S. Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of protein. Asian Pacific Journal of Tropical Biomedicine. 2012;2(1):S178–80.
16. Mizushima Y, Kobayashi M. Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins. Journal ofPharmacy and Pharmacology. 2011;20(3):169–73.
17. Adekola MB, Areola JO, Fagbohun OF, Asaolu FT, Ogundepo GE, Fajobi AO, et al. In-vitro antioxidant and anti-inflammatory activities of ethanol stem-barkextract of Blighia sapida K.D. Koenig. Journal of Pharmaceutical Analysis. 2022;12(2):350–54.
Published
30/11/2023
Statistics
281 Views | 148 Downloads
Citatons
How to Cite
Abraham P, E., Francis, F., Nair, P. R., Raj, A., Rajan, R., Nair, A. K., & R, B. (2023). In vitro screening of icacinaceous plants indigenous to kerala. Journal of Innovations in Applied Pharmaceutical Science (JIAPS), 8(3-S), 51-58. https://doi.org/10.37022/jiaps.v8i3-S.527
Section
Research Article(S)