Influence of variation extraction methods (classical procedure) for antibacterial activity of Rarugadong (Dioscorea pyrifolia Kunth.) tuber
Abstract
Drug resistance due to widespread abuse and excessive use of antibiotics has become an increasingly serious problem, making the development of alternative antibacterial a very urgent problem. The aim of this study was to define whether suitable extraction methods, such as maceration, reflux and soxhlet method. In this study, rarugadong (Dioscorea pyrifolia Kunth.) tuber extract, was extracted by maceration, reflux and soxhlet method respectively using methanol as solvent. Phytochemical screening carried out on rarugadong (Dioscorea pyrifolia Kunth.) tuber in simplicia powder and each extract. Antibacterial activity was determined using agar well diffusion method. Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 were used as test bacterial in this study. Phytochemistry screening results of simplicia and extract gave the same result, it was showed positive results against the class of chemical compounds alkaloids, flavonoids, glycosides, saponins, triterpenoids and tannins, anthraquinone glycosides and cyanogenic glycosides. Antibacterial activity of methanol extract from maceration, reflux and soxhlet methods showed inhibition for both bacteria (Staphylococcus aureus and Escherichia coli) from the measurement of inhibition diameter in each extract concentration.
Keywords:
Secondary metabolites, antibacterial, Dioscorea pyrifolia Kunth, screening phytochemistry, maceration, reflux, soxhlet
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References
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28. T.P. Tim Cushnie, Benjamart Cushnie and Andrew J. Lamb. Alkaloids: An overview of their antibacterial, antibiotic-enhancing and antivirulence activities. International Journal of Antimicrobial Agents 2014; 44: 377-386.
29. Karlina Garcia-Sosa, Ninibe Villarreal-Alvarez, Petra Lubben and Luis M. Pena-Rodriguez. Chrysophanol, an antimicrobial anthraquinone from the root extract of Colubrina greggii. J. Mex. Chem. Soc 2006; 50(2): 76-78.
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32. D.S. Ogunleye and S.F. Ibitoye. Studies of antimicrobial activity and chemical constituents of Ximenia americana. Tropical Journal of Pharmaceutical Research 2003; 2(2): 239-241.
33. A. Doss, H. Mohammed Mubarack and R. Dhanabalan. Antibacterial activity of tannins from the leaves of Solanum trilobatum Linn. Indian Journal of Science and Technology 2009; 2(2): 41-43.
34. Rebamang A. Mosa, MandlakayiseL. Nbleko, Thandeka V. Dladla and Anoly R. Opoku. Antibacterial activity of two triterpenes from stem bark of Protorhus longifolia. Journal of Medicinal Plant Research 2014; 8(18): 686-702.
2. Nofiani, R., Siti Nurbetty and Ajuk Sapar. Antimicrobial activity of methanol extract with sponge based from lemukutan island, West Kalimantan. E-Journal of Ilmu dan Teknologi Kelautan Tropis 2009; 1(2): 33-41.
3. Rika Pratiwi Rijayanti. Antibacterial activity of ethanol extract of Mangga Bacang (Mangifera foetida L.) leaves against Staphylococcus aureus by in Vitro. Jurnal Mahasiswa PSPD FK Universitas Tanjungpura 2014; 1(1):1-18.
4. Refdanita, Maksum R, Nurgani, Endang. The pattern of germ sensitivity to antibiotics in the intensive care room at Fatmawati Jakarta Hospital in 2001-2002. Journal of Makara kesehatan 2004; 8(2): 41-48. Jakarta.
5. Sonibare, Mubo Adeola. Rebecca Busayo Abegunde. In vitro antimicrobial and antioxidant analysis of Dioscorea dumetorum (Kunth) Pax and Dioscorea hirtiflora (Linn.) and their bioactive metabolites from Nigeria. J. Appl. Bosci 2012; 3583-3590.
6. Chandra, Subhash, Sarla Saklani, Abhay P. Mishra. In vitro antimicrobial activity of garhwal himalaya medicinal plant Dioscorea deltoidea Tuber. International Journal of Herbal Medicine 2013; 1(4): 67-70.
7. Sandhu, P.S. Baljinder Singh, Vikas Gupta, Parveen Bansal, Dharimendra Kumar. Potential herbs used in ocular diseases. Journal of Pharmaceutical Science and Research 2011; 3 (4): 1127-1140.
8. James Hamuel Doughari. Phytochemicals: extraction methods, basic structures and mode of action as potential chemotherapeutic agents, phytochemicals - A global perspective of their role in nutrition and health. Dr Venketeshwer Rao (Ed.). ISBN: 978-953-51-0296-0, In Tech, Available from:http://www.intechopen.com/books/phytochemicals-a-global-perspective-of-their-role-in-nutrition-andhealth/phytochemicals-extraction-methods-basic-structures-and-mode-of-action-as potentialchemotherapeutic. 2012.
9. Sabri Fatima Zohra, Belarbi Meriem, Sabri Samira, Alsayadi Muneer M.S. Phytochemical screening and identification of some compounds from mallow. J. Nat. Prod. Plant Resour. 2012; 2(4): 512-516.
10. K. Sahira Banu and Dr. L. Cathrine. General techniques involved in phytochemical. Analysis International Journal of Advanced Research in Chemical Science 2015; 2(4):25-32.
11. Sylvia V Egan, Hockhin Yeohand, J Howard Bradbury. Simple picrate paper kit for determination of the cyanogenic potential of cassava flour. J Sci Food Agric. 1998; 76:39-48.
12. Jagessar R.C. and R. Allen. Phytochemical Screening And Atomic Absorption Spectroscopic Studies Of Solvent Type Extract From Leaves of Terminalia catappa, (Almond). Academic Research International, Part-1: Natural and Applied Sciences 2012. 3(3): 17-26.
13. Prasant Tiwari, Bimlesh Kumar, Mandeep Kaur, Gurpreet Kaur, Harleen Kaur. Phytochemical screening and extraction: A Review. Internationale Pharmaceutica Sciencia 2011; 1(1): 98-106.
14. Yang Lin Yeo, Yin Yin Chia, Chin Hong Lee, Heng Sheng Sow and Wai Sum Yap. Effectiveness of maceration periods with different extraction solvents on in-vitro antimicrobial activity from fruit of Momordica charantia L. Journal of Applied Pharmaceutical Science 2014; 4(10): 016-023.
15. Ho, Y.M. Wan Amir Nizam, W.A. and Wan Rosli, W.I. Antioxidative activities and polyphenolic content of different varieties of malaysian young corn ear and cornsilk. Sains Malaysiana 2016; 45(2): 195-200.
16. Himanshu Aggarwal, Jayashri Ghosh, Alka Rao, and Vinod Chhokar. Evaluation of root and leaf extracts of Glycrriza glabra for antimicrobial activity. Journal of Medical and Bioengineering 2015; 4(1): 81-85.
17. Ncube N.S., Afolayan A.J. and Okoh A.I. Assessment techniques of antimicrobial properties of natural compounds of plant origin: Current methods and future trends. African Journal of Biotechnology 2008; 7(12):1797-1806.
18. Hossein Jahangirian, MD Jelasharon, Mohd Halim Shah Ismail, Rushanak Rafiee-Moghaddam, Leili Afsah-Hejri, Yadollah Addollahi, Majid Rezayi and Nazanin Vafaei. Well diffusion method for evaluation of antibacterial activity of copper phenyl fatty hydroxamate synthesized from canola and palm kernel oils. Digest Journal of Nanomaterials and Biostructures 2013; 8(3): 1263-1270.
19. Mohamed Elshikh, Syed Ahmed, Scott Funston, Paul Dunlop, Mark McGaw, Roger Marchant, Ibrahim M. Banat. Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants. Biotechnol Lett 2016; 38: 1015-1019.
20. A Eralp Akca, Gulcin Akca, Fulya Toksoy Topcu, Enis Macit, Levent Pikdoken and I. Serif Ozgen. The comparative evaluation of the antimicrobial effect of propolis with chlorhexidine against oral pathogens: An In vitro study. Biomed Research International 2016; 2016. Article ID 3627463: 1-8.
21. Anoma Chandrasekara and Thamilini Josheph Kumar. Roots and tuber crops as functional foods: a review on phytochemical constituents and their potential health benefits. International Journal of Food Science 2016; 2016. Article ID 3631647: 1-15.
22. A.B. Abd El-Aziz and H.H Abd El-Kalek. Antimicrobial proteins and oil seeds from pumpkin (Cucurbita moschata). Nature and Science 2011; 9(3): 105-119.
23. Prabha Shetty, N. Supraja, M. Garud and T.N.V.K.V. Prasad. Synthesis, characterization and antimicrobial activity of Alstonia scholaris bark-extract-mediated silver nanoparticles. J Nanostruct Chem 2014; 4: 161-170.
24. Simona Conea, Laurian Vlase and Ioan Chirila. Comparative study on the polyphenols and pectin of three eryngium species and their antimicrobial activity. Cellulose Chemistry and Technology 2016; 50(3-4): 473-481.
25. Saeed A. Hayek, Rabin Gyawali and Salam A. Ibrahim. Antimicrobial natural products. formatex – microbial pathogens and strategies for combating them: Science, Technology and Education 2013; 910-921.
26. N.S. Radulovic, P.D. Blagojevic, Z.Z. Stojanovic-Radic and N.M. Stojanovic. Antimicrobial plant metabolites: structural diversity and mechanism of action. Current Medicinal Chemistry 2013; 20(7): 932-952.
27. El Zawane Kamarudin, Qamar Uddin Ahmed, Abul Bashar Mohammed Helaluddin, Zuvairea Nazren Mohd Sirajudin, Ahmad Jalal Khan Chowdhury. Studies on bactericidal efficacy of pumpkin (Cucurbita moschata Duchesne) Peel. Journal of Coastal Life Medicine 2014; 2(2): 146-153.
28. T.P. Tim Cushnie, Benjamart Cushnie and Andrew J. Lamb. Alkaloids: An overview of their antibacterial, antibiotic-enhancing and antivirulence activities. International Journal of Antimicrobial Agents 2014; 44: 377-386.
29. Karlina Garcia-Sosa, Ninibe Villarreal-Alvarez, Petra Lubben and Luis M. Pena-Rodriguez. Chrysophanol, an antimicrobial anthraquinone from the root extract of Colubrina greggii. J. Mex. Chem. Soc 2006; 50(2): 76-78.
30. Soetan K.O, Oyekunle M.A., Aiyelaagbe O.O. and Fafunso M.A. Evaluation of the antimicrobial activity of saponins extract of Sorghum bicolor L. Moench. African Journal of Biotechnology 2006; 5(23): 2405-2407.
31. T.P. Tim Cushnie and Andrew J. Lamb. Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents 2005; 26: 343-356.
32. D.S. Ogunleye and S.F. Ibitoye. Studies of antimicrobial activity and chemical constituents of Ximenia americana. Tropical Journal of Pharmaceutical Research 2003; 2(2): 239-241.
33. A. Doss, H. Mohammed Mubarack and R. Dhanabalan. Antibacterial activity of tannins from the leaves of Solanum trilobatum Linn. Indian Journal of Science and Technology 2009; 2(2): 41-43.
34. Rebamang A. Mosa, MandlakayiseL. Nbleko, Thandeka V. Dladla and Anoly R. Opoku. Antibacterial activity of two triterpenes from stem bark of Protorhus longifolia. Journal of Medicinal Plant Research 2014; 8(18): 686-702.
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31/03/2019
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Mierza, V., Rosidah, Haro, G., & Suryanto, D. (2019). Influence of variation extraction methods (classical procedure) for antibacterial activity of Rarugadong (Dioscorea pyrifolia Kunth.) tuber. Journal of Innovations in Applied Pharmaceutical Science (JIAPS), 4(1), 01-06. Retrieved from https://www.saapjournals.org/index.php/jiaps/article/view/197
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