In vitro cloning of dang sam (Codonopsis pilosula (Franch) Nannf)

Đoan Trong Đức; Bui Thanh Hoa; Tran Van Minh

Đoan Trong Đức Kon Tum Center for Scientific Application and Technology Transfer.
Bui Thanh Hoa International University, Vietnam National University HCM.
Tran Van Minh International University, Vietnam National University HCM.

Abstract

Sterilization of Dang sam (Campanumoea pilosula (Franch) Nannf) cutting explants by HgCl₂ 0.1% in 5 minutes reach 58.3% survivals. The MS medium supplemented with 2.4D 2 mg/L and TDZ 0.1 mg/L was favored for callus induction on cutting surface after 30 days. Callus was proliferated on MS-1/2 medium supplemented with 2.4D 2 mg/L and TDZ 0.1 mg/L after 8 weeks. Callus cluster of 5 mm diameter were regenerated on MS medium supplemented with BA 2 mg/L and NAA 0.5 mg/L initiated multiple shoots after 60 days. Multiple shoots were divided into shoot clusters having diameter of 5 mm with 3-5 shoots/cluster. Shoot clusters were cultured on multiplication medium of MS supplemented with BA 2 mg/L and NAA 0.5 mg/L initiated to new shoots and the shoots were bigger after 20 days. Shoots were divided into 1 cm length cuttings with 2 leaves and cultured on MS medium supplemented with BA 0.5 mg/L and IBA 0.7 mg/L for regeneration to whole plantlets having 4-5 leaves, 6-10 roots, 4-6 cm root length reaching survivals of 85.07% and being ready for acclimatization environment. Sand substrate was favored for acclimatization in the first 10 days of ex vitro culture and mix-soil substrate was favored for plantlet growth and development afterward.

Keywords: Acclimatization, callus, multiple shoots, regeneration, sterilization

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