Formulation, development and evaluation of polyherbal gel for topical infection

Deepak Soni Deepak Soni; Mukesh Kumar Patel Mukesh Kumar Patel; Ashish Manigauha Ashish Manigauha; Arun Pandey Arun Pandey

Deepak Soni Mittal Institute of Pharmacy, Bhopal-462038, MP (India)
Mukesh Kumar Patel Mittal Institute of Pharmacy, Bhopal-462038, MP (India)
Ashish Manigauha Mittal Institute of Pharmacy, Bhopal-462038, MP (India)
Arun Pandey Mittal Group of Institutions, Bhopal-462038, MP (India)

Abstract

Objective: The purpose of this research was to formulate polyherbal gel, a combination of Acorus calamus, Annona squamosa and Chenopodium album and to evaluate anti-acne activity against Propionibacterium acnes.

Methods: Topical gel formulations were prepared using carbopol 940 as a gelling agent. Test parameters for topical gel include organoleptic, extrudability, spreadability and pH. The anti-acne activity test of Acorus calamus, Annona squamosa and Chenopodium album using agar well diffusion method was carried out.

Results: The results of this study showed that Acorus calamus, Annona squamosa and Chenopodium album extracts had anti-acne activity. Anti-acne activity combination of Acorus calamus, Annona squamosa and Chenopodium album extract is synergistic. The results of preliminary phytochemical investigation showed the presence of carbohydrates, cardiac glycosides, phenols and flavonoids. Quantitative analysis of total phenolic and flavonoid content compounds showed high content of phenolic compounds and flavonoids. Other parameters such as spreadability, pH, viscosity and extrudability were found to be well within the limits.

Conclusion: Hydroalcoholic extracts of Acorus calamus, Annona squamosa and Chenopodium album can be formulated as hydrogels with satisfactory physicochemical parameters for anti-acne activity.

Keywords: Acorus calamus, Annona squamosa, Chenopodium album, Polyherbal gel, Anti-acne activity, Propionibacterium acnes

Downloads

Download data is not yet available.

References

1. Cogen AL, Nizet V, Gallo RL. Skin microbiota: A source of disease or defence? Br J Dermatol 2008; 158: 442-455.
2. Dessinioti C, Katsambas AD. The role of Propionibacterium acnes in acne pathogenesis: facts and controversies. Clin Dermatol 2010; 28: 2–7.
3. De PV, Mark N, Abel BL. Synthesis and fungicidal, insecticidal activity of new amide derivatives of piperine. Pest Management Sci 2000; 56: 168-174.
4. Jain A, Amita J. Tridax procumbens (L.): A weed with immense medical impotance: A review. Int J Pharma Bio Sci 2012; 3: 544-552.
5. Chandra D, Prasad K. Phytochemicals of Acorus calamus (Sweet flag). J Med Plant Stud 2017; 5: 277-281
6. Bhattacharya A, Chakraverty R. The pharmacological properties of Annona squamosa Linn: A Review. Int J Pharm Eng 2016; 4: 692-699.
7. Dai Y, Ye WC, Wang ZT, Matsuda H, Kubo M, But PPH. Antipruritic and antinociceptive effects of Chenopodium album L. in mice. J Ethnopharmacol 2002; 81: 245-250.
8. Kumar R, Mishra AK, Dubey NK, Tripathi YB. Evaluation of Chenopodium ambrosioides oil as a potential source of antifungal, antiaflatoxigenic and antioxidant activity. Int J Food Microbio 2006; 115: 159-164
9. Trease GE, Ewan WC. Pharmacognocy. 1313th edn Bailliere Tindal, London, 1989: 176-180
10. Wagner H, Baldt S, Zgainski EM. Plant drug analysis. New York, Berlin, Springer, 1996.
11. Wagner H, Bladt S, Zgainski EM. Plant drug analysis-A Thin Layer Chromatography Atlas. Springer-Verlag, Berlin, 1984
12. Kim DO, Jeong SW, Lee CY. Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem 2003; 81: 321-326.
13. Katasani D. Phytochemical screening, quantitative estimation of total phenolic, flavanoids and antimicrobial evaluation of Trachyspermum ammi. J Atom Molecul 2011; 1: 1-8.
14. Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. American J Clin Path 1966; 45: 493-496.
15. Akerele O. Medicinal plants and primary health cares: An agenda for action. Fitoterapia 1988; 59: 355–363.
16. Tewari D, Pandey HK, Sah AN, Meena HS, Manchanda A. Pharmacognostical and biochemical investigation of Ocimum kilimandscharicum plants available in western Himalayan region. Asian J Plant Sci Res 2012; 2: 446-451.