Targeting Oxidative Stress with Carrot Seed Extracts: A Phytochemical and In Vitro Antioxidant Study

Biswanath Prusty; Santanu Kumar Hotta; Jochhana Rani Bhuyan; Chayakanta Behera

doi:10.46956/ijihd.v10i2.703

Biswanath Prusty Department of Pharmaceutical Analysis, College of Pharmaceutical Sciences, Berhampur, Ganjam, Odisha, pin- 760002, India
Santanu Kumar Hotta Department of Pharmaceutical Chemistry, College of Pharmaceutical Sciences, Berhampur, Ganjam, Odisha, pin- 760002, India
Jochhana Rani Bhuyan Department of Pharmaceutics, College of Pharmaceutical Sciences, Berhampur, Ganjam, Odisha, pin- 760002, India
Chayakanta Behera Department of Pharmaceutics, College of Pharmaceutical Sciences, Berhampur, Ganjam, Odisha, pin- 760002, India

DOI https://doi.org/10.46956/ijihd.v10i2.703

Abstract

The present study aimed to investigate the in vitro antioxidant potential and phytochemical constituents of Daucus carota seed extracts. Various solvent fractions (petroleum ether, diethyl ether, chloroform, ethyl acetate, methanol, and water) were analyzed for radical scavenging activity using DPPH, nitric oxide, hydrogen peroxide, and total antioxidant capacity assays. The ethyl acetate extract exhibited the most potent DPPH scavenging activity (IC₅₀ = 1.38 ± 0.01 μg/mL), while the aqueous extract showed the highest nitric oxide scavenging effect (IC₅₀ = 3.20 ± 0.05 μg/mL). Phytochemical analysis revealed the presence of alkaloids, flavonoids, phytosterols, proteins, and carbohydrates across different extracts. These findings suggest that Daucus carota seeds are a promising source of natural antioxidants.

Keywords: Daucus carota, antioxidant, phytochemical screening, IC₅₀, free radicals, DPPH, nitric oxide

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