Anti-stress activity of different extracts of Caralluma fimbriata in wistar rats

Somayeh Afsah Vakili Somayeh Afsah Vakili; Syed Fayazuddin Syed Fayazuddin

Somayeh Afsah Vakili Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore-560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India
Syed Fayazuddin Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore Department of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, BangaloreDepartment of Pharmacology, Visveswarapura Institute Pharmaceutical Sciences, Bangalore-560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India 560070, Karnataka, India

Abstract

Objective: The current investigation was planned to assess adaptogenic activity of aqueous and ethanol extracts of Caralluma fimbriata

Methods: Anti-stress of aqueous and ethanol extracts of Caralluma fimbriata was appraised by inducing stress in rats through the forceful swimming. Biochemical parameters such as 6-β-OH-cortisol, urinary vanillymandelic acid (VMA), homovanillic acid (HVA), ascorbic acid and 5-hydroxyindoleacetic acid (5HIAA) were estimated to analyze the anti-stress activity. Aqueous and ethanol extracts of Caralluma fimbriata were administrated orally at dose of 200 and 400 mg/kg body weight one h before the induction of stress to prevent stress-induced urinary biochemical changes in a dose dependent manner.

Results: Nevertheless, in all groups which were treated with aqueous and ethanol extracts of Caralluma fimbriata, there was no statistically difference between level of 6-β-OH-cortisol, urinary vanillymandelic acid (VMA), homovanillic acid (HVA), ascorbic acid and 5-hydroxyindoleacetic acid (5HIAA) as compared to normal animals.

Conclusion: The present investigation indicated that aqueous and ethanol extract of Caralluma fimbriata have potential anti-stress activity.

Keywords: Adaptogenic activity, Caralluma fimbriata, Biochemical parameters

Downloads

Download data is not yet available.

References

1. Stavri M, Gibbon S. The antimycobacterial constituents of dill (Anthem graveolens). Phytother Res 2005; 19: 938- 941.
2. Ral D, Bhatla G, Sen T, Palt G. Comparative study of perturbations of peripheral markers in different stressors in rats. Can J Physiol Pharmacol 2003; 81: 1139-1146.
3. Chrousos GP, Gold PW. The concepts of stress and stress system disorders: Overview of physical and behavioural homeostasis. J Am Med Assoc 1992; 267: 1244-1252.
4. Eliyahu SB, Liebeskind RY, Taylor AN, Gale RP. Stress increases metastatic spread of mammary tumor in rats: Evidence for mediation by immune system. Brain Behav Immun 1991; 5: 193-205.
5. Cheng GJ, Morrow-Tech JL, Beller DI, Levy EM, Black PH. Immunosuppression in mice induced by cold water stress. Brain Behav Immun 1990; 4: 278-291.
6. Wagner H, Norr H, Winterhoff H. Plant adaptogens. Phytomed 1994; 1: 63-78.
7. Rege NN, Thatte UM, Dhanukar SA. Adaptogenic properties of six rasayana herbs used in Ayurvedic medicine. Phytother Res 1999; 13: 275-291.
8. Kuriyan R, Raj T, Srinivas SK, Vaz M, Rajendran R, Kurpad AV. Effect of Caralluma fimbriata extract on appetite, food intake and anthropometry in adult Indian men and women. Appetite 2007; 48: 338-344.
9. Saivasnthi V, Gowthamigoud, Swathi K, Avanapu SR. Evaluation of Caralluma fimbrita for analgesic, anti-inflammatory and anxiolytic activities. Int J Pharma 2011; 1: 40-45.
10. Sreemantula S, Boini KM, Nammi S. Reserpine methonitrate, a novel quaternary analogue of reserpine augments urinary excretion of VMA and 5-HIAA without affecting HVA in rats. BMC Pharmacol 2004; 4: 1-7.
11. Roe JH, Kuether CA. The determination of ascorbic acid in whole blood and urine through the 2, 4- Dinitrophenylhydrazine derivative of dehydroascorbic acid. J Biol Chem 1943; 147: 399-407.
12. Chang KC, Yang JJ, Wang-Hsu, Chiu TH, Hsu FC. Epigallocatechin-3-gallate inhibits the spontaneous firing of rat locus coreleus neuron. Neurosci Lett 2009; 452: 141-145.
13. Resine TD, Sourbie P, Artaud F, Glowinski J. Involvement of lateral habenula-dorsal raphe neurons in the differential regulation of striatal and nigral serotonergic transmission in cats. J Neurosci 1982; 2: 1062-1071.
14. Rajaram K, Suresh KP. Phytochemical studies and GC-MS analysis of Caralluma fimbriata wall. Int J Pharm Res Dev 2011; 3: 105-110.
15. Patil RA, Jagadle SC, Kasture SB. Antihyperglycemic,
antistress and nootropic activity of roots of Rubia cordifolia Linn. Indian J Exp Biol 2006; 44: 987-992.
16. Samson J, Devi S, Ravindran R, Manohar S. Biogenic amine changes in brain regions and attenuating action of Ocimum sanctum in noise exposure. Pharmacol Biochem Behav 2006; 83: 67-75.
17. Singh VB, Onaivi ES, Phan TH, Boadle-Biber MC. The increase in rat cortical and midbrain tryptophan hydroxylase activity in response to acute or repeated sound stress are blocked by bilateral lesions to the central nucleus of the amygdala. Brain Res 1990; 530: 49-53.