Evaluation of anti-inflammatory activity of hydroalcoholic extract of black tea leaves in-vitro

Abstract

Objective: The objective of present investigation was to determine the activity of hydroalcoholic extract of tea leaves against protein denaturation and inflammation.

Methods: The method employed for the investigation comprised of a reaction mixture containing egg albumin, phosphate buffer and varying concentrations of hydroalcoholic extract. Individual mixtures were incubated at 37 ºC for 15 min followed by heating at 70 ºC for 5 min. Absorbance of different mixtures was measured at 660 nm. Indomethacin was taken as standard drug. Percent inhibition was calculated.

Results: Percentage inhibition of hydroalcoholic extract and indomethacin was found to be increasing with increase in their respective concentrations (250-1250 μg/ml).

Conclusion: The present study provides useful information about the anti-inflammatory activity of hydroalcoholic extract of black tea leaves. Hydroalcoholic extract showed significant decrease in inflammation, although the action was found to be less than that of standard drug.