Antioxidant activity, total phenolic and flavonoid content of selected medicinal plants from Gorkha district, Nepal
Abstract
Objective: To determine the antioxidant activity, total phenolic and flavonoid content of the methanolic extracts of Fritillaria cirrhosa, Rosa sericea, Neopicrorhiza scrophulariflora and Juniperus squamata.
Methods: Antioxidant ability of the extracts was evaluated using 2, 2 diphenyl-1-picrylhydrazil (DPPH) as the source of free radical and butylated hydroxyanisole and vitamin C as reference compounds. Total phenolic content of the sample was estimated by the Folin-Ciocalteu method and total flavonoid content by the aluminium chloride method.
Results: The extracts of J. squamata contained highest phenolic content whereas R. sericea contained highest amount of flavonoids. F. cirrhosa has negligible amount of total phenolic and flavonoids. Antioxidant activity, as determined by DPPH assay revealed that J. squamata and R. sericea had stronger activity.
Conclusion: J. squamata and R. sericea possess higher phenolic and flavonoid content and strong antioxidant activity and could be useful as natural antioxidants.
Keywords:
DPPH, Phenolic Content, Flavonoid Content, Medicinal Plants
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References
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2. Manandhar NP. Plants and people of Nepal, Timber Press INC, USA; 2002.
3. Annonymous 2007a. Gift of the Himalayas, Non-timber forest products of the sacred Himalayan landscape- Nepal, WWF Nepal; 2007.
4. Annonymous 2007b. Medicinal plants of Nepal, Bulletin of the department of medicinal plants number 3, Ministry of forest and soil conservation, Department of plant resources, Government of Nepal, Thapathali, Kathmandu;1997.
5. Baral SR, Kurmi PP. A Compendium of medicinal plants in Nepal. Mrs Rachana Sharma, Kathmandu, Nepal; 2006.
6.Ghimire SK, Lama YC, Tripathi GR, Schmitt S, Thomas YA. Conservation of plant resources, community development and training in applied ethnobotany at Shey-Phoksundo national park and its buffer zone, Dolpa. Report series No 41. WWF Nepal, Kathmandu, Nepal; 2001.
7. Kahkonen MP, Hopia AI, Vuorela HJ, Rauha JP, Pihlaja K, Kujala TS, Heinonen, M. Antioxidant activity of plant extracts containing phenolic compounds. J Agric Food Chem 1999; 47: 3954-3962.
8. Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med 1996; 20: 933-956.
9. Halliwell B, 1990. How to characterize a biological antioxidant. Free Rad Res Commun 1990; 9:1–32.
10. Skrovankova S, Misurcova L, Machu L. Antioxidant activity and protecting health effects of common medicinal plants, Adv Food Nutr Res 2012; 7: 75-139.
11. Stainton A. Flowers of the Himalayas-A supplement. Oxford University Press, New Delhi, India, 1988.
12. Polunin O, Stainton A. Flowers of the Himalayas, Oxford University Press, Delhi-Bombay-Calcutta-Madras, India,1984.
13. Shimada K, Fujikawa K, Yahara K, Nakamura T. Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J Agric Food Chem 1992; 40: 945-948.
14. Iqbal S, Bhanger MI, Akhtar M, Anwar F, Ahmed KR, Anwer T. Antioxidant properties of methanolic extracts from leaves of Rhazya stricta. J Med Food 2006; 9: 270-275.
15. Woisky R, Salatino A. Analysis of propolis: Some parameters and procedures for chemical quality control. J Apicul Res 1998; 37: 99-105.
16. Nantitanon W, Yotsawimonwat S, Okonogi S. Factors
influencing antioxidant activities and total phenolic content of guava leaf extract. LWT - Food Sci Tech 2010; 43:1095-1103.
17. Li S, Li SK, Gan RY, Song FL, Kuang L, Li HB. Antioxidant capacities and total phenolic contents of infusions from 223 medicinal plants. Ind Crops Prod 2013; 51: 289-298.
18. Lesjak MM, Ivana N, Beara N, Orcic DZ, Ristic JD, Anackov GT, Bozin BN, Mimica-Dukic NM. Chemical characterisation and biological effects of Juniperus foetidissima Willd. 1806. LWT - Food Sci Tech 2013; 53: 530-539.
19. Orhan N, Orhan IE, Ergun F. Insights into cholinesterase inhibitory and antioxidant activities of five Juniperus species. Food Chem Toxicol 2011; 49: 2305–2312.
20. Chaouche TM, Haddouchi F, Ksouri R, Medini F, Atik-Bekara F. In vitro evaluation of antioxidant activity of the hydro-methanolic extracts of Juniperus oxycedrus subsp. Oxycedrus. Phytothérapie 2013; 11: 244-249.
21. Sahreen S, Khan MR, Khan RA. Evaluation of antioxidant activities of various solvent extracts of Carissa opaca fruits. Food Chem 2010; 122:1205-1211.
22. Cai YZ, Sun M, Luo Q, Corke H. Structure–radical scavenging activity relationships of phenolic compounds from traditional Chinese medicinal plants. Life Sci 2004; 78: 2872-2888.
23. Terpinc P, Cehb B, Natasa Poklar Ulrih NP, Abramovic H. Studies of the correlation between antioxidant properties and the total phenolic content of different oil cake extracts, Ind Crops Prod 2012; 39: 210-217.
24. Sulaiman SF, Md. Yusoff NA, Eldeen IM, Seow EM, Sajak AAB, Supriatno, Ooi KL. Correlation between total phenolic and mineral contents with antioxidant activity of eight Malaysian bananas (Musa sp.) J Food Comp Anal 2011; 24: 1-10.
Published
31/08/2017
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How to Cite
[1]
L. R. B. Lok Ranjan Bhatt and O. B. Om Basukala, “Antioxidant activity, total phenolic and flavonoid content of selected medicinal plants from Gorkha district, Nepal”, Int J Indig Herb Drug, pp. 5-8, Aug. 2017.
Section
Research Articles
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