Analysis of fatty acids levels of freeze-dried termite queen Macrotermes gilvus Hagen using gas chromatography-mass spectrometry

Yohannes Alen Yohannes Alen; Orindia Suarmin Orindia Suarmin; Lakmi Nurul Suci Lakmi Nurul Suci; Harrizul Rivai Harrizul Rivai

Yohannes Alen Faculty of Pharmacy, Andalas University, Padang 25163, West Sumatera, Indonesia
Orindia Suarmin Faculty of Pharmacy, Andalas University, Padang 25163, West Sumatera, Indonesia
Lakmi Nurul Suci Faculty of Pharmacy, Andalas University, Padang 25163, West Sumatera, Indonesia
Harrizul Rivai Faculty of Pharmacy, Andalas University, Padang 25163, West Sumatera, Indonesia

Abstract

Objective: The objective of the study was to evaluate the fatty acids composition of freeze dried termite queen Macrotermes gilvus Hagen by gas chromatography (GC) combined with mass spectrometry (MS) and the most important physical-chemicals parameters.

Methods: Freeze dried termite queen oil that was used for analysis has been obtained by soxhlet extraction method. It is known that individual fatty acids can be identified by GC because of their different retention times, the samples of freeze dried termite queen oil were esterified to bring them into a vaporous phase, transforming the fatty acid from freeze dried termite queen oil into fatty acids methyl esters.

Results: The results showed that the major component of freeze dried termite queen oil was oleic acid (omega-9), linoleic acid (omega-6) and EPA (omega-3) contents 32812.70 mg/100 g (32.81 %), 345.15 mg/100 g (0.34 %) and 72.20 mg/100 g (0.07%) respectively.

Conclusion: It can be concluded that freeze dried termite queen oil is an excellent source of essential fatty acids omega-9 (oleic acid).

Keywords: Fatty Acids, Freeze Dried, Dried Termite Queen Oil, Macrotermes gilvus, Omega-9

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