Anti-oxidant activity determination of Clerodendron infortunatum Linn by in vivo method

Mathew George Mathew George; Lincy Joseph Lincy Joseph; Leena PN Leena PN

Mathew George Professor, Department of Pharmacology, Pushpagiri College of Pharmacy, Thiruvalla, Kerala, India
Lincy Joseph Professor, Department of Pharmaceutical Chemistry, Pushpagiri College of Pharmacy, Thiruvalla, Kerala, India
Leena PN Associate Professor, Department of Pharmacognosy, Pushpagiri College of Pharmacy, Thiruvalla, Kerala, India

Abstract

Objective: To evaluate the anti-oxidant activity determination of Clerodendron infortunatum linn by in vivo method.

Methods: Serum was analyzed for various biochemical parameters like serum glutamic oxaloacetic (SGOT), serum glutamic pyruvic transaminase (SGPT) activities and alkaline phosphatase. The total protein concentration and total bilirubin were also measured. All the analyses were performed. Estimation of lipid peroxidation (LPO), enzymic (CAT,) non-enzymic (GSH) antioxidant system by the livers were quickly removed, weighed and homogenized in phosphate buffer (0.1 M, pH 7.4) The homogenate was centrifuged at (1000 rpm , 15 min) to remove debris. The supernatant was used to assay the LPO, CAT and GSH activities. The percentage inhibition was calculated using the equation: %Inhibition =A0-A1 /A0.

Results: Ethanolic extract of Clerodendron infortunatum Linn in the dose of 200 mg/kg, p.o.,400mg/kg has improved the glutathione, SOD, catalase and peroxidase levels significantly, which were comparable with standard.

Conclusion: In the present study,plant Clerodendron infortunatum, were selected based on their ethnomedical uses. Ethanolic extract of Clerodendron infortunatum Linn has improved the glutathione, SOD, catalase and peroxidase levels significantly, which were comparable with standard.

Keywords: Anti-oxidant activity, Clerodendron infortunatum, Root, Liv 52, In vivo method

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