Analysis of amino acids levels of freeze-dried termite queen Macrotermes gilvus Hagen

  • Yohannes Alen Faculty of Pharmacy, Andalas University, Padang 25163, West Sumatera, Indonesia
  • Lakmi Nurul Suci` Faculty of Pharmacy, Andalas University, Padang 25163, West Sumatera, Indonesia
  • Orindia Suarmin Faculty of Pharmacy, Andalas University, Padang 25163, West Sumatera, Indonesia
  • Harrizul Rivai Faculty of Pharmacy, Andalas University, Padang 25163, West Sumatera, Indonesia

Abstract

Objective: Termites (Macrotermes gilvus Hagen) are the social insects with colonies divided into ‘castes’ that include workers, soldiers, winged adults, a queen and a king. Queen of termites were analyzed for amino acid content. Methods: Amino acid analyses were carried out on the part reproductive termites to determine the nutrient composition. The queen termites were collected from palm plantation, Taluak Kuantan, Riau, Indonesia. They were opened from nest, freeze-dried to fine powder. Amino acids were determined using ultra pressure liquid chromatography (UPLC). Results: Freeze-dried termites queen showed that crude protein contain the essential amino acid. The highest content of amino acid was lysine, while the least content of amino acid was tryptophan. Conclusion: The queen of termites was the nutrient-rich insects that can supply adequate quantities of the various amino acids for pharmaceutical nutrient.

Keywords: Termites, UPLC, Lysine, Tryptophan, Macrotermes gilvus

Downloads

Download data is not yet available.

References

1. Food-Info.net. Classes of edible insects. http://www.food-info.net/uk/products/insects/ classes.htm, accessed on 15 June 2017.
2. Alamu OT, Amao AO, Nwokedi CI, Oke OA, Lawa IO. Diversity and nutritional status of edible insects in Nigeria: A review. Int J Biodiv Cons 2013; 5: 215-222.
3. Alen Y, Suci LN, Suarmin O. Analysis levels of fatty acids from freeze-dried termite queen Macrotermes gilvus Hagen using GC-MS and antihyperlipidemia test. Unpublished data, 2016.
4. Alen Y, Yuliandra Y, Adhitya R. Acute toxicity and sub acute toxicity assay of freeze dried termite queen Macrotermes gilvus Hagen. National seminar and workshop on recent developments of pharmaceutical sciences and clinics VI, Padang, Indonesia. 2016.
5. Alen Y, Aldi Y, Rahmawati R. Immunomodulatory activity of freeze dried termite queen Macrotermes gilvus Hagen. Unpublished data, 2016.
6. Hirs CWH. Performic acid oxidation. Methods Enzymol 1967; 11: 197-199.
7. Hugli TE, Moore S. Determination of the tryptophan content of proteins by ion exchange chromatography of alkaline hydrolysates. J Biol Chem 1972; 10: 247: 2828-2834.
8. Bidlingmeyer BA, Cohen SA, Tarvin TL. Rapid analysis of amino acids using pre-column derivatization. J Chromatogr B: Biomed Sci Appl 1984; 336: 93-104.
9. Hariharan M, Naga S, VanNoord T. Systematic approach to the development of plasma amino acid analysis by high-performance liquid chromatography with ultraviolet detection with precolumn derivatization using phenyl isothiocyanate. J Chromatogr B: Biomed Sci Appl 1993; 17; 621: 15-22.
10. DeFoliart GR. Insect fatty acids: similar to those of poultry and fish in their degree of unsaturation, but higher in the polyunsaturates. The Food Insects Newsletter 1991; 4: 1-4.
11.Ajayi OE. Biochemical analyses and nutritional content of four castes of subterranean termites, Macrotermes subhyalinus (Rambur) (Isoptera: Termitidae): differences in digestibility and anti-nutrient contents among castes. Int J Biol 2012; 4: 54-59.
12. Ntukuyoh AI, Udiong DS, Ikpe E, Akpakpan AE. Evaluation of nutritional value of termites (Macrotermes bellicosus): soldiers, workers, and queen in the Niger delta region of Nigeria. Int J Food Nutr Saf 2012; 1: 60-65.
13. Suzuki A. On the insect-eating habits among wild chimpanzees living in the savanna woodland of western Tanzania. Primates. 1966; 7: 481-487.
14. Siulapwa N, Mwambungu A, Lungu E, Sichilima W. Nutritional value of four common edible insects in Zambia. Int J Sci Res 2014; 3: 876-884
15. Afiukwa JN, Okereke C, Odo MO. Evaluation of proximate and mineral contents of termite (Trinervitermes germinatus) from Abakaliki and Ndieze izzi, Ebonyi state, Nigeria. Am J Food Nutr 2013; 3: 98-104.
16. Adepoju OT, Omotayo OA. Nutrient composition and potential contribution of winged termites (Marcrotermes bellicosus Smeathman) to micronutrient intake of consumers in Nigeria. Br J Appl Sci Technol 2014; 4: 1149-1158.
17. FAO. Forestry Paper: Edible insects: future prospects for food and feed security. FAO, Rome. 2013.
Published
30/06/2017
Statistics
131 Views | 141 Downloads
Citatons
How to Cite
[1]
Y. A. Yohannes Alen, L. N. S. Lakmi Nurul Suci, O. S. Orindia Suarmin, and H. R. Harrizul Rivai, “Analysis of amino acids levels of freeze-dried termite queen Macrotermes gilvus Hagen”, Int J Indig Herb Drug, pp. 1-5, Jun. 2017.
Section
Research Articles