Evaluation of antibacterial property of anthyocyanin extracted from brassica oleracea against gram positive and gram negative bacteria by using erythromycin as a standard drug.
Abstract
Anthocyanin was extracted from Brassica oleracea and identified by UV and TLC.The benzene test initially helps us to identify Anthocyanin in residue. Crystals were purified by recrystallization from ether. Acetone –petroleum ether extraction resulted in higher crude Anthocyanin yield than hexane extraction The compounds were tested against two microorganisms namely E. coli (gram-ve) and bacillus (gram+ve) and the compounds showed significant activity against two species. Test sample one Showed significant activity with zone of inhibition (18.21mm). When compared to standard Erythromycin with zone of inhibition (20.83mm) at 100,200,400mg/ml against E. coli organism. Test sample two Showed significant activity with zone of inhibition (15.56mm) when compared to standard Erythromycin with zone of inhibition at (100,200,400mg/ml) against Bacillus.
Keywords:
Brassica Oleracea, Anthocyanin, Erythromycin, Bacillus
Downloads
Download data is not yet available.
References
1. Werner Mantele, ErhanDeniz. UV-VIS absorption spectroscopy. SpectrochimicaActa Part A:Molecular and BiomolecularSpectoscopy 173;965-968,2017.
2. Sara J Palmer,BJagannadhaReddy,Ray L Frost.Characterisation of red mud by UV-vis-NIR spectroscopy.SpectrochimicaActa Part A:Molecular and Biomolecular Spectroscopy 71 (5),1814-1818,2009.
3. ReinhardLange,ClaudeBalny.UV-visible derivative spectroscopy under high pressure.Biochimica et BiophysicaActa-Protein Structure and Molecular Enzymology 1595 (1-2),80-93,2002.
4. YI Yu, GuangXiong,canLi,Feng-ShouXiao.Characterisation of aluminosilicate Zeolites by UV Spectroscopy.Microporous and mesoporous materials 46 (1)23-34,2001.
5. GV Andrievsky,VKKlochkov,ABBordyuh,GIDovbeshko.Comparative analysis of two aqueous-collidal solutions of C60 fullerene with help of UV-vis Spectroscopy.Chemical physics Letters 364 (1-2),8-17,2002.
6. B Roig,CGonzalez,OThomas.Monitoring of phenol photodegradation by ultraviolet spectroscopy.SpectrochimicaActa Part A:Molecular and Biomolecular Spectroscopy 59 (2),303-307,2003.
7. Alexei NikolaevichPestryakov,Vitali P Petranovskii,AndrevKrvazhov.Study of copper nanoparticles formation on supports of different nature by UV-Vis diffuse reflectance spectroscopy.Chemical physics Letters 385 (3-4),173-176,2004.
8. SockalingamAnbazhagan,NarayanareddyIndumathy.pitchaimuthuShanmugapandivan.Simultaneous quantification of Stavudine,lamivudine by UV Spectroscopy, reverse phase HPLC and HPTLC in tablets.Journal of pharmaceutical and biomedical analysis 39 (3-4),801-804,2005.
9. D.Bonazzi,RGotti,VAndrisano,VCavrini.Analysis of ACE inhibitors in pharmaceutical dosage forms by derivative UV spectroscopy and liquid chromatography (HPLC).Journal of pharmaceutical and biomedical analysis 16 (3),431-438,1997.
10. Singh, A., Srinivasan, A.K., Chakrapani, L.N. and Kalaiselvi, P., 2019. LOX-1, the common therapeutic target in hypercholesterolemia: a new perspective of antiatherosclerotic action of aegeline. Oxidative medicine and cellular longevity, 2019.
11. M Monica Giusti,RonaldE.Characterisation and measurement of anthocyanins by UV-visible spectroscopy.Current protocols in food analytical chemistry,Wiley Online library, F1.2.1-F1.2.13,2001.
12. Konatham Teja Kumar Reddy, & M. Akiful Haque. (2022). Develop and validate a highly sensitive method for the estimation of Molnupiravir in rat plasma by high-performance liquid chromatography-tandem mass spectroscopy and its application to pharmacokinetic studies. Journal of Pharmaceutical Negative Results, 28–34. https://doi.org/10.47750/pnr.2022.13.S01.0
13. Konatham Teja Kumar Reddy, Penke Vijaya Babu, Rajinikanth Sagapola, & Peta Sudhakar. (2022). A REVIEW OF ARTIFICIAL INTELLIGENCE IN TREATMENT OF COVID-19. Journal of Pharmaceutical Negative Results, 254–264. https://doi.org/10.47750/pnr.2022.13.S01.31
14. Singh, A., Gowtham, S., Chakrapani, L.N., Ashokkumar, S., Kumar, S.K., Prema, V., Bhavani, R.D., Mohan, T. and Sathyamoorthy, Y.K., 2018. Aegeline vs Statin in the treatment of Hypercholesterolemia: A comprehensive study in rat model of liver steatosis. Functional Foods in Health and Disease, 8(1), pp.1-16.
15. Konatham Teja Kumar Reddy, Kumaraswamy Gandla, Penke Vijaya Babu, M Vinay Kumar Chakravarthy, Pavuluri Chandrasekhar, & Rajinikanth Sagapola. (2022). A CRITICAL REVIEW ON BIOANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF FEW ONCOLOGY DRUGS BY USING LC-MS-MS. Journal of Pharmaceutical Negative Results, 16–27. https://doi.org/10.47750/pnr.2022.13.S01.03
16. Reddy, K. T. K., & Haque, M. A. (2022). Bioanalytical method development and validation of atrasentan in human plasma using verapamil as internal standard by liquid chromatography coupled with tandem mass spectrometry. International Journal of Health Sciences, 6(S8), 625–638. https://doi.org/10.53730/ijhs.v6nS8.10470
17. Konatham Teja Kumar Reddy et.al High Performance Liquid Chromatography for The Simultaneous Estimation of Anti-Ulcer Drugs in Pharmaceutical Dosage Form, journal of Positive School Psychology, Vol. 6, No. 9, 4524-452
18. Reddy KTK, Haque MA. Development and Validation of a High Throughput Lc-Ms/MS Method for Quantitation of Ipilimumab in Human Plasma. International Journal of Pharmaceutical Quality Assurance. 2022;13(3):303-307
19. Singh, A., Kumar, A. and Kalaiselvi, P., 2018. Aegeline, targets LOX1, the receptor for oxidized LDL to mitigate hypercholesterolemia: a new perspective in its anti-atherosclerotic action. Free Radical Biology and Medicine, 128, p.S41.
20. Teja Kumar Reddy Konatham, M. Anuradha (2020), a stability indicating method development and validation of Telmisartan and Nifedipine in pure form using RP-HPLC. International Journal of Pharmaceutical, Biological and Chemical Sciences, 9(3): 36-44
21. Teja Kumar Reddy Konatham, Satyanarayana Reddy K., Anuradha Manipogo, a Review on viruses that originated from china; Sars, mers and covid-19 World Journal of Pharmaceutical Research, Vol 9, Issue 5, 2020, 2010-2015.
22. Teja Kumar Reddy Konatham et al, A Systematic Review on Method Development and Validation of Few Antiviral Drugs by Using RP-HPLC. Ijppr.Human, 2021; Vol. 21 (3): 651-661.
23. Singh, A., 2022. Role of microbial metabolites in cardiovascular and human health. In Microbiome, Immunity, Digestive Health and Nutrition (pp. 137-148). Academic Press.
24. Konatham Teja Kumar Reddy and Kumaraswamy Gandla. Novel Vesicular Drug Delivery Systems Proniosomes. Pharm Res 2022, 6(3): 000272.
25. Yelampalli SR, Gandla K, Reddy KT, Ibrahim AE, El Deeb S. Determination of Sodium, Potassium, and Magnesium as Sulfate Salts in Oral Preparations Using Ion Chromatography and Conductivity Detection. Separations. 2023 Feb 1;10(2):99.
26. Asha Deepti et al / Stability Indicating Rp-Hplc Method for Assay of Dexamethasone In Its FormulationNEUROQUANTOLOGY | SEPTEMBER 2022 | VOLUME 20 | ISSUE 11 | PAGE 80-86| DOI: 10.14704/NQ.2022.20.11. NQ66009
27. Singh, A., 2022. Hyperlipidemia in cardiovascular health and digestion. In Nutrition and Functional Foods in Boosting Digestion, Metabolism and Immune Health (pp. 141-150). Academic Press.
28. Deepti, A., & Sahithi, A. (2022). A sensitive bioanalytical method development and validation of afatinib in human plasma by LC-ESI-MS/MS. International Journal of Health Sciences, 6(S5), 1736– 1384. https://doi.org/10.53730/ijhs.v6nS5.9522
29. Kumar, K. P., Marathakam, A., Patnaik, S., Kumar, S., Sahithi, A., Priya, D. K. S., & Dogra, P. (2022). An insight of development and validation of bioanalytical method in the reference of anticancer drugs by using LC-MS/MS. International Journal of Health Sciences, 6(S3), 6349–6361. https://doi.org/10.53730/ijhs.v6nS3.7454
30. Alapati Sahithi, B. Parijatha, D. Santhoshi Priya, Krishna Mohan Chinnala, Method Development and validation of Metformin by using RP-HPLC, World Journal of Pharmaceutical Research, 2277-7105, 6(8); August,2017
31. Sahithi et al, Stability indicating reverse-phase high-performance liquid chromatography method development and validation of Imatinib, International Journal of Research and Analytical Reviews, Vol 7, Issue 3, 322-333, September 2020. DOI: 10.20959/wjpr20178-9069
2. Sara J Palmer,BJagannadhaReddy,Ray L Frost.Characterisation of red mud by UV-vis-NIR spectroscopy.SpectrochimicaActa Part A:Molecular and Biomolecular Spectroscopy 71 (5),1814-1818,2009.
3. ReinhardLange,ClaudeBalny.UV-visible derivative spectroscopy under high pressure.Biochimica et BiophysicaActa-Protein Structure and Molecular Enzymology 1595 (1-2),80-93,2002.
4. YI Yu, GuangXiong,canLi,Feng-ShouXiao.Characterisation of aluminosilicate Zeolites by UV Spectroscopy.Microporous and mesoporous materials 46 (1)23-34,2001.
5. GV Andrievsky,VKKlochkov,ABBordyuh,GIDovbeshko.Comparative analysis of two aqueous-collidal solutions of C60 fullerene with help of UV-vis Spectroscopy.Chemical physics Letters 364 (1-2),8-17,2002.
6. B Roig,CGonzalez,OThomas.Monitoring of phenol photodegradation by ultraviolet spectroscopy.SpectrochimicaActa Part A:Molecular and Biomolecular Spectroscopy 59 (2),303-307,2003.
7. Alexei NikolaevichPestryakov,Vitali P Petranovskii,AndrevKrvazhov.Study of copper nanoparticles formation on supports of different nature by UV-Vis diffuse reflectance spectroscopy.Chemical physics Letters 385 (3-4),173-176,2004.
8. SockalingamAnbazhagan,NarayanareddyIndumathy.pitchaimuthuShanmugapandivan.Simultaneous quantification of Stavudine,lamivudine by UV Spectroscopy, reverse phase HPLC and HPTLC in tablets.Journal of pharmaceutical and biomedical analysis 39 (3-4),801-804,2005.
9. D.Bonazzi,RGotti,VAndrisano,VCavrini.Analysis of ACE inhibitors in pharmaceutical dosage forms by derivative UV spectroscopy and liquid chromatography (HPLC).Journal of pharmaceutical and biomedical analysis 16 (3),431-438,1997.
10. Singh, A., Srinivasan, A.K., Chakrapani, L.N. and Kalaiselvi, P., 2019. LOX-1, the common therapeutic target in hypercholesterolemia: a new perspective of antiatherosclerotic action of aegeline. Oxidative medicine and cellular longevity, 2019.
11. M Monica Giusti,RonaldE.Characterisation and measurement of anthocyanins by UV-visible spectroscopy.Current protocols in food analytical chemistry,Wiley Online library, F1.2.1-F1.2.13,2001.
12. Konatham Teja Kumar Reddy, & M. Akiful Haque. (2022). Develop and validate a highly sensitive method for the estimation of Molnupiravir in rat plasma by high-performance liquid chromatography-tandem mass spectroscopy and its application to pharmacokinetic studies. Journal of Pharmaceutical Negative Results, 28–34. https://doi.org/10.47750/pnr.2022.13.S01.0
13. Konatham Teja Kumar Reddy, Penke Vijaya Babu, Rajinikanth Sagapola, & Peta Sudhakar. (2022). A REVIEW OF ARTIFICIAL INTELLIGENCE IN TREATMENT OF COVID-19. Journal of Pharmaceutical Negative Results, 254–264. https://doi.org/10.47750/pnr.2022.13.S01.31
14. Singh, A., Gowtham, S., Chakrapani, L.N., Ashokkumar, S., Kumar, S.K., Prema, V., Bhavani, R.D., Mohan, T. and Sathyamoorthy, Y.K., 2018. Aegeline vs Statin in the treatment of Hypercholesterolemia: A comprehensive study in rat model of liver steatosis. Functional Foods in Health and Disease, 8(1), pp.1-16.
15. Konatham Teja Kumar Reddy, Kumaraswamy Gandla, Penke Vijaya Babu, M Vinay Kumar Chakravarthy, Pavuluri Chandrasekhar, & Rajinikanth Sagapola. (2022). A CRITICAL REVIEW ON BIOANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF FEW ONCOLOGY DRUGS BY USING LC-MS-MS. Journal of Pharmaceutical Negative Results, 16–27. https://doi.org/10.47750/pnr.2022.13.S01.03
16. Reddy, K. T. K., & Haque, M. A. (2022). Bioanalytical method development and validation of atrasentan in human plasma using verapamil as internal standard by liquid chromatography coupled with tandem mass spectrometry. International Journal of Health Sciences, 6(S8), 625–638. https://doi.org/10.53730/ijhs.v6nS8.10470
17. Konatham Teja Kumar Reddy et.al High Performance Liquid Chromatography for The Simultaneous Estimation of Anti-Ulcer Drugs in Pharmaceutical Dosage Form, journal of Positive School Psychology, Vol. 6, No. 9, 4524-452
18. Reddy KTK, Haque MA. Development and Validation of a High Throughput Lc-Ms/MS Method for Quantitation of Ipilimumab in Human Plasma. International Journal of Pharmaceutical Quality Assurance. 2022;13(3):303-307
19. Singh, A., Kumar, A. and Kalaiselvi, P., 2018. Aegeline, targets LOX1, the receptor for oxidized LDL to mitigate hypercholesterolemia: a new perspective in its anti-atherosclerotic action. Free Radical Biology and Medicine, 128, p.S41.
20. Teja Kumar Reddy Konatham, M. Anuradha (2020), a stability indicating method development and validation of Telmisartan and Nifedipine in pure form using RP-HPLC. International Journal of Pharmaceutical, Biological and Chemical Sciences, 9(3): 36-44
21. Teja Kumar Reddy Konatham, Satyanarayana Reddy K., Anuradha Manipogo, a Review on viruses that originated from china; Sars, mers and covid-19 World Journal of Pharmaceutical Research, Vol 9, Issue 5, 2020, 2010-2015.
22. Teja Kumar Reddy Konatham et al, A Systematic Review on Method Development and Validation of Few Antiviral Drugs by Using RP-HPLC. Ijppr.Human, 2021; Vol. 21 (3): 651-661.
23. Singh, A., 2022. Role of microbial metabolites in cardiovascular and human health. In Microbiome, Immunity, Digestive Health and Nutrition (pp. 137-148). Academic Press.
24. Konatham Teja Kumar Reddy and Kumaraswamy Gandla. Novel Vesicular Drug Delivery Systems Proniosomes. Pharm Res 2022, 6(3): 000272.
25. Yelampalli SR, Gandla K, Reddy KT, Ibrahim AE, El Deeb S. Determination of Sodium, Potassium, and Magnesium as Sulfate Salts in Oral Preparations Using Ion Chromatography and Conductivity Detection. Separations. 2023 Feb 1;10(2):99.
26. Asha Deepti et al / Stability Indicating Rp-Hplc Method for Assay of Dexamethasone In Its FormulationNEUROQUANTOLOGY | SEPTEMBER 2022 | VOLUME 20 | ISSUE 11 | PAGE 80-86| DOI: 10.14704/NQ.2022.20.11. NQ66009
27. Singh, A., 2022. Hyperlipidemia in cardiovascular health and digestion. In Nutrition and Functional Foods in Boosting Digestion, Metabolism and Immune Health (pp. 141-150). Academic Press.
28. Deepti, A., & Sahithi, A. (2022). A sensitive bioanalytical method development and validation of afatinib in human plasma by LC-ESI-MS/MS. International Journal of Health Sciences, 6(S5), 1736– 1384. https://doi.org/10.53730/ijhs.v6nS5.9522
29. Kumar, K. P., Marathakam, A., Patnaik, S., Kumar, S., Sahithi, A., Priya, D. K. S., & Dogra, P. (2022). An insight of development and validation of bioanalytical method in the reference of anticancer drugs by using LC-MS/MS. International Journal of Health Sciences, 6(S3), 6349–6361. https://doi.org/10.53730/ijhs.v6nS3.7454
30. Alapati Sahithi, B. Parijatha, D. Santhoshi Priya, Krishna Mohan Chinnala, Method Development and validation of Metformin by using RP-HPLC, World Journal of Pharmaceutical Research, 2277-7105, 6(8); August,2017
31. Sahithi et al, Stability indicating reverse-phase high-performance liquid chromatography method development and validation of Imatinib, International Journal of Research and Analytical Reviews, Vol 7, Issue 3, 322-333, September 2020. DOI: 10.20959/wjpr20178-9069
Published
19/02/2023
Statistics
362 Views | 185 Downloads
Citatons
How to Cite
[1]
A. K. Tallam, A. Sahithi, and M. V. Nuli, “Evaluation of antibacterial property of anthyocyanin extracted from brassica oleracea against gram positive and gram negative bacteria by using erythromycin as a standard drug.”, Int J Indig Herb Drug, vol. 8, no. 1, pp. 1-6, Feb. 2023.
Issue
Section
Research Articles
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright © Author(s) retain the copyright of this article.
.