Effect of Curcuma longa extract on Candida albicans adhesion to heat cure acrylic resin denture material: An in-vitro study

Niharika Yamala Niharika Yamala; Vandana Raghunath Vandana Raghunath

Niharika Yamala Department of Oral and Maxillofacial Pathology, Narayana Dental College, Nellore- 524003, Andhra Pradesh (India)
Vandana Raghunath Department of Oral and Maxillofacial Pathology, Narayana Dental College, Nellore- 524003, Andhra Pradesh (India)

Abstract

Objective: Adherence of Candida species to oral surfaces is regarded as a prerequisite for successful colonisation of the oral cavity. Acrylic dentures act as reservoirs and increases the risk of Candida colonisation, as denture stomatitis is the frequently reported complication among denture wearers. Commercial denture cleansers have been studied but the use of natural products is greatly advantageous. Curcumin, the major active compound of Curcuma longa (turmeric) noted for its potent antifungal action has been shown in-vitro to inhibit adherence of Candida species to human buccal epithelial cells. Hence, to know the effect of curcumin on Candidal adherence to acrylic surfaces, this study was undertaken. Methods: 15 square blocks (1 cm x 1 cm x 2 mm) of heat cure acrylic resin were prepared, autoclaved and incubated in a suspension of Candida albicans (600x105 CFU/ml). A set of 5 were transferred to test tubes containing 2 ml of 0.1%, 0.2% and 0.3% curcumin solution and left for 8 h. Each block was imprinted onto separate SDA agar plate and incubated for 24 h at 37 ºC. Then the CFU’s were counted and statistically analyzed. Results: 0.3% of curcumin solution was found to be effective against Candida albicans adherence onto heat cure acrylic denture material with a statistically significant p value < 0.05. Conclusion: Curcumin proved to be effective, thus paving the way to be used as an effective and a natural denture cleanser.

Keywords: Curcumin, Candida albicans, Heat cure acrylic resin, Denture cleanser

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